Fig. 6

Fig. 6

Dysregulated embryonal globin expression in Cdan^ΔEry erythroblasts. qRT-PCR was performed with peripheral blood at E9.5 and E11.5, and normalized to Rplp0. The relative expression of embryonic β-like globins (A) and embryonic α-like globin (B) are presented as mean ± SEM (n = 4–6 for each genotype). The Student’s t-test was applied for statistical analysis. Asterisks indicate significant difference from the Control. *p < 0.01; **p < 0.001. (C) α-globin switch is delayed in Cdan^ΔEry erythroblasts. Expression of ζ and α was determined by RT-qPCR. The data are displayed as the fraction of total α-like globin (mζ+mα) expression. Embryonic day is indicated. (D) Expression of εy, and βh1 was determined by RT-qPCR. The data are displayed as the fraction of total embryonal β-like globin (mεy+mβh1) expression.