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Figure 4. Differential spatiotemporal deposition of C-dots in regenerating bones. (A) Diagram of regenerating fins used in analysis. Amputated fish were injected with saline (control) or C-dots at the indicated times. One hour after injection, fins were re-amputated and processed for cryosectioning. Black arrow indicates amputation site. Red and blue lines indicate the approximate site of sections in regenerating and non-regenerating bones, respectively. (B) Sagittal sections of regenerated and non-regenerated caudal fins ordered in distal (younger) and proximal (older) direction (position corresponding to red and blue lines in A, respectively). Areas of C-dot deposition are green. Areas in white box are shown magnified in the bottom panels. Scale bar is 100 µm for low and 20 µm for high magnification images.