Figure 1

Figure 1

Analysis of shoaling in Neu1-KO and WT zebrafish. (a–c) Analysis of the behavior of Neu1-KO zebrafish (6 months) in the normal tank for 5 min. (a) Tracking. (b) Swimming distance. (c) Average swimming speed. WT, wild-type and KO, Neu1-KO zebrafish. n = 7 for each group. (d–h) Shoaling of Neu1-KO zebrafish. Five fish were set in the experimental tank, and their average inter-fish distance was immediately estimated (d, without acclimation) . After 30 min acclimation in the test tank, inter-fish distances were evaluated again (f, with acclimation). Closed blue triangles and red squares with single line indicate WT and KO, respectively. (e) Average fish number in the top area for 5 min. (g) Average distance between nearest and (h) farthest neighbors analyzed for 5 min in WT and Neu1-KO zebrafish during the shoaling test without acclimation. Results are shown as means ± standard deviation. All values were compared using Mann–Whitney U test. *p < 0.05, **p < 0.01. n.s., not significant.