ZFIN Image: Follain et al., 2021, Figure 1

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Figure Caption

Figure 1

Flow favors expression of gene program driving vasculature remodeling, including VEGFRs. (A) Experimental setup: microfluidic experiment for RNA sequencing. (B) Results from global Gene Ontology (GO) analysis on ‘biological process’, showing most significantly impacted GO class. (C) Fold changes heatmap, based on GO class: Angiogenesis, showing significantly upregulated genes in flow condition compared to no flow. (D) Western blot quantification validating some of VEGFR RNAseq data. (E) Representative image of flow vs no flow immunofluorescence labelling of FLT1, KDR and FLT4 (green) with DAPI (blue). (F) Quantification associated with D and E, showing increased expression and signal intensity of VEGFRs. WB: N = 5, Mann Whitney test, IF: N = 4 with min 5 fields/exp, Mann Whitney test.

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