ZFIN Image: Mauri et al., 2021, Fig. 7

Image

Figure Caption

Fig. 7

In the absence of arteries, cells fail to sprout in grb2b mutants. a–c Confocal projections of plcγ-1 MO injected embryos. All vessels are shown in green (fli:GFP). d Sprout quantification at 48hpf of embryos from a tabula rasa in-cross, injected with a plcγ-1 MO. plcγ-1 MO injection inhibits primary sprouting, allowing quantification/visualization of secondary sprouts in absence of arteries. The number of lympho-venous sprouts is significantly decreased in tabula rasa mutants. wt: n = 4; het: n = 32; mut: n = 24. ****Between wt and mut: P value < 0.0001 (Mann–Whitney); ****Between het and mut: P value < 0.0001 (Mann–Whitney); ns not significant. MO morpholino. Scale bars: 50 µm. Data in d are mean ± sd

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Angiogenesis