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Figure 8

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Publication: Ishii et al., 2021 - Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens
All Figures: Figures for Ishii et al., 2021

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Figure 8

Fine 3D imaging at cellular level. (a) Mouse cerebral cortex was stained by Golgi silver impregnation method. (b) Block-face image of the stained cortex. (c, d) The minimum intensity projection (MinIP) image and volume-rendered image were generated from 201 block-face images with voxel size 0.9 × 0.9 × 1 µm. (e) Mouse liver were injected with black ink via bile duct. (f) A piece of the liver were imaged using CoMBI-S, and a block-face image is shown. (g, h) 3D volume-rendered images using 401 block-face images with voxel size of 4 × 4 × 1 µm (g) or 1 × 1 × 1 µm (h) shows the biliary tree. Bars: 100 µm (a–d, f–h), 5 mm (e).

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