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Figure 2

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Publication: Ishii et al., 2021 - Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens
All Figures: Figures for Ishii et al., 2021

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Figure 2

Correlation between 3D image and sections of juvenile zebrafish. A juvenile zebrafish (30 dpf) is sliced at a thickness of 5 µm using CoMBI-S. (a, b) 3D volume-rendered image using 568 block-face images. (c–e) Examples of block-face images. (f–h) Sections were collected at the positions corresponding to (c–e), and stained with hematoxylin and eosin. (i–k) Higher magnification of (f–h). 1: lateral forebrain bundle, 2: ventral thalamus, 3: dorsal thalamus, 4: telencephalic ventricle, 5: habenula, 6: zona limitans intrathalamica, 7: preoptic region, 8: optic nerve, 9: retina, 10: mandibular cartilage, 11: palatoquadrate ceratohyal, 12: pharynx, 13: quadrate, 14: cornea, 15: vitreous body, 16: lens, 17: retinal ganglion cell layer, 18: inner plexiform layer, 19: inner nuclear layer, 20: outer plexiform layer, 21: outer nuclear layer, 22: inner ear, 23: brainstem, 24: cranial base, 25: gill, 26: gas bladder, 27: liver, 28: intestine, 29: spinal cord, 30: vertebral body, 31: notochord remnant. Bars in (a), (f) 500 µm and (i) 250 µm.

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