Figure 3 supplement 1

Figure 3 supplement 1

(A) GO analysis of biological processes shows enrichment of cytoskeletal related genes (in red) in snai1b^-/- hearts compared with snai1b^+/+ hearts. (B, C) Schematic of gfp and snai1b mRNA injections into one-cell stage embryos. snai1b and desmb mRNA levels were analysed in injected embryos at 4.5 hpf. Relative mRNA levels of snai1b are significantly increased, whereas desmb mRNA levels are decreased at 4.5 hpf. n = 4 biological replicates, 30 embryos each. (D) In vitro luciferase assay in HEK293T cells after co-transfection of pGL4.14-luciferase and pGL4.14–800 bp desmb-luciferase with pTol2-CMV-snai1b or alone. Reduced luciferase activity was detected when pTol2-CMW-snai1b was co-transfected with pGL4.14–800 bp desmb-luciferase. Plot values represent means ± S.D.; p-values determined by Student’s t-test (C) or one-way ANOVA followed by multiple comparisons with Dunn test (D). FC: fold change. All Ct values are listed in Supplementary file 2.