ZFIN Image: Gentile et al., 2021, Figure 1 supplement 3

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Figure Caption

Figure 1 supplement 3 Wild-type like cardiomyocyte (CM) numbers in <italic toggle='yes'>snai1b</italic> mutants.

(A–A”’) TUNEL assay analysis indicates that extruding CMs in snai1b^-/- embryos are not apoptotic. Maximum intensity projection of Tg(myl7:BFP-CAAX) snai1b^+/+ (A) and snai1b^-/- (A’–A”) hearts at 50 hpf. Close-up of extruding CM (arrowhead) labelled with DAPI, but not with TUNEL (A”). (B, B’) Maximum intensity projections of Tg(myl7:H2B-GFP) snai1b^+/+ (B) and snai1b^-/- (B’) hearts at 52 hpf. (C) The total number of CMs does not change significantly in snai1b^-/- hearts (n = 12) compared with snai1b^+/+ hearts (n = 12). Plot values represent means ± S.D.; p-value determined by Student’s t-test. Scale bars: 20 µm (A–A’, B–B’); 10 µm (A’’). V: ventricle; A: atrium; n: number of embryos.

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Acknowledgments

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