Figure 3 MBP-CC1 in vitro experiments. (A) Caco-2 cells were treated with MBP-CC1, MBP-CC2, MBP-CC3 or MBP-COC. No addition of proteins or MBP-CPE was used for controls. The graph represents the change in TEER compared to the control (no addition of protein). Following treatment, TEER was monitored for 24 h (intervals of 0, 1, 2, 4, 8, 12, and 24 h). Three experiments are averaged in the graph (±SD). (B) Cal27 cells were treated with EGF, MBP-CC1, MBP-COC, MBP-CPE or MBP-CPE(m19). After 24 h of treatment, cells were prepared for the proliferation assay (ATPlite, Perkin Elmer). Proliferation is reported as the average of 4 separate experiments (±SD). For statistical analysis, we employed t-test, and the asterisk represents a statistical significance (p < 0.001).
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