ZFIN Image: Cunningham et al., 2021, Fig. 1

Image

Figure Caption

Fig. 1 ATAC-Seq of zebrafish melanoma tumors identifies <italic>peak5</italic> as an active neural crest enhancer.

a Schematic for obtaining zebrafish melanoma tumor cells for ATAC-Seq. b Regions of open chromatin surrounding the sox10 locus. Peak numbers are annotated below. MP minimal promoter. c Schematic for screening putative enhancers for activity in NCCs and melanoma. dpeak5-driven EGFP expression is mosaically present in NCCs (arrowheads) in F0 injected embryos, as indicated by co-localization with crestin:mch at 1 dpf. e The sox10 minimal promoter in F0 embryos is active in both NCCs (arrowheads) and the CNS (asterisk). f F0 embryos injected with a negative control (~76 kb upstream of the sox10 TSS) exhibit only limited expression of EGFP not localized to NCCs. Insets show enlarged region above yolk extension.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Commun Biol