ZFIN Image: Orr et al., 2021, FIGURE 2

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FIGURE 2

Endocannabinoid signaling pathway is socially regulated and its modulation of M-cell excitability is status-dependent. (A) qPCR gene expression analysis of ECS signaling molecules. Data of dominants and subordinates normalized to control isolates (n = 6 pairs; control isolates n = 6; Kruskal–Wallis test, *p < 0.05). (B) Western blot analysis of dagl-α and cb1 receptor. Each protein was tested concurrently with β-actin as a control. Protein expression of dominants and subordinates was normalized to communal controls as a ratio (illustrated values below each band); C, communals; D, dominants; S, subordinates. Bar graphs represent average % change in protein concentration of four replicates of samples each consisting of 10 brains normalized to WT communals.

Acknowledgments

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