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FIGURE 5 Stat3 inhibitor S3I-201 suppresses RG proliferation after stab wound injury in adult optic tectum. (A–C) Representative images of proliferative RG (BLBP+ PCNA+) in injured optic tectum treated with 1% DMSO (A), 10 μM S3I-201 (B), or 100 μM S3I-201 (C) at 1 dpi. (A’–C’) Magnified images of the boxed area in each image. White arrowheads indicate BLBP+ PCNA+ cells and dashed lines indicate the area injured by needle insertion. Scale bar: 50 μm in (A–C) and (A’–C’). (D) Schematic diagram of drug administration. (E) Quantification of proliferative RG (BLBP+ PCNA+) treated with 1% DMSO, 10 μM S3I-201 (n = 4), or 100 μM S3I-201 (n = 5) at 1 dpi. Statistical analyses were performed using one-way ANOVA with Tukey’s post hoc test. (F) Quantification of proliferative cells except RG (BLBP-PCNA+) treated with 1% DMSO, 10 μM S3I-201 (n = 4), or 100 μM S3I-201 (n = 5) at 1 dpi. Statistical analyses were performed using one-way ANOVA with Tukey’s post hoc test.