ZFIN Image: Cooper et al., 2021, Figure 5

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Figure Caption

Figure 5

gfp mRNA and vtg mRNA levels in ERE-TG Casper zebrafish. Levels of gfp mRNA and vtg mRNA were measured in transgenic zebrafish exposed to EE2 (10 ng/L) a WwTW effluent (50% or 100%) or system water control during the developmental periods of (A) 0-90 days, (B) 21-60 days, and (C) 60-90 days. Data are reported as mean ± SE and expressed as fold-change compared with the control. Relative mRNA expression was determined as the ratio or target gene mRNA/rpl8 mRNA. Asterisks denote a significant difference (*p ≤ 0.05, **p ≤ 0.01, *** p ≤ 0.001) compared with control. Within groups, different letters denote a significant difference (p ≤ 0.05).

Acknowledgments

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