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Figure 1
Design of the zebrafish effluent exposure experiment. Exposures were conducted to a system water control (SWC), EE2 (10 ng/L) or WwTW effluent concentrations of 100% or 50% for the different developmental life stages indicated. Exposures are indicated with solid horizontal lines with dashed lines indicating fish maintenance in clean water. There were three replicate tanks per treatment. The time points for measurement of the different endpoints are indicated. From 0-4 dpf, embryos/larvae were housed in 500 mL glass dishes without any water flow at a density of 50 fish per dish and kept in an incubator at 28 °C. From 5-21 dpf, fish were kept in 3.5 L nursery tanks placed within the 20 L glass tanks and then subsequently transferred to the 20 L tanks at 21 dpf.