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Fig. 10 (A) Whole-mount RNA in situ hybridization for notch3 and msx3 in 30 hpf Mnpc2+/m and MZnpc2m/m embryos. Expression of both notch3 and msx3 was observed mainly in the head area and was reduced in MZnpc2m/m embryos compared with Mnpc2+/m embryos. The numbers of embryos examined showing the represented staining over the total numbers of embryos examined are indicated in each image (n=3 experiments). Scale bars: 200 μm (30 hpf); 100 μm (dorsal). (B) Magnifications of the otic vesicles shown in A. Both notch3 and msx3 were expressed along the lining of the otic vesicle in Mnpc2+/m embryos. However, their expression was diminished around the otic vesicle in MZnpc2m/m embryos (arrowheads). Scale bars: 100 μm.