Fig 6

Fig 6

(A) Crossing schemes for mapping variation in two independent families, BC_a and BC_b (see main text). (B–C) Log odds scores of example QTL (BC_a) for melanophore spacing (nearest neighbor distance, log₂(DV:AP variation), and number of melanophore elements across 25 chromosomes. Variation segegrating between species in blue and within D. kyathit in red. Dashed lines indicate q = 0.05 false discovery thresholds and asterisks indicate peak LOD scores. (E) Summary of regions associated with pattern variation and size in the same family referenced in B–C (BC_a), and defined by F_ST values for phenotypically extreme individuals from a second family (BC_b; see main text). Regions highlighted have peaks (q<0.05, LOD≥4) marked by vertical bars and widths defined by positions 1.5 LOD lower than peaks. Percents of phenotypic variance explained by QTL (%var) are indicated at right. Small, vertical hash marks on chromosomes denote BC_a maternal D. kyathit marker loci and positions of several genes having roles in pigment pattern formation in D. rerio are indicated as well.