ZFIN Image: Shimizu et al., 2021, Figure 3

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Figure Caption

Figure 3

Measurement of candidate gene expression in LIPUS-treated zebrafish and medaka. Zebrafish and medaka tail bones were fractured and stimulated with LIPUS for 20 min every day. On days 1 and 7 after the fracture, RNA was extracted from the fin rays. (a) Immunity-, Secretion-, and (b) Transcription-related genes were measured (n = 3). For medaka, Btg2 and Cited2 couldn’t design qPCR primers because reference sequences were not available (N/A) in the public database. Values presented are the mean ± SEM and the significance of differences was determined using Student’s t-test. * p < 0.05; ** p < 0.01.

Acknowledgments

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