ZFIN Image: Magnussen et al., 2021, Figure 2

Image

Figure Caption

Figure 2

Structural characterization of MDM2^f and MDM2^z. (a–c) Crystal structures of MDM2^z (a; purple/light purple), MDM2^f (b; blue/light blue) and MDM2^h (c; orange/light orange, PDB: 6SQO). Zinc ions are shown as gray spheres. The diameter of the dimer was calculated by measuring the distance between the Cα atoms of R471 (human nomenclature) of both protomers. (d) Superimposition of (a–c) in ribbon form. (e) Reduced SDS-PAGE showing autoubiquitination reactions catalyzed by GST-MDM2 variants using fluorescently-labeled Ub and visualized by an Odyssey CLx Imaging System (top panel) or stained with Coomassie Blue (bottom panel). (f) Crystal structure of the MDM2^f-UbcH5B–Ub complex. UbcH5B and Ub are colored in cyan and yellow, respectively. MDM2^f is colored as in b. (g,h) Close-up views of the key interactions between MDM2^f and UbcH5B involving MDM2^f’s I431 (g) and R470 (h),

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Biol.