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Figure 4 Activation of LEN is attenuated by hemodynamic force alteration. (A) Schematic timeline diagram of MO injection and MTZ treatment. (B,C) Confocal stack projections of ablated Tg(vmhc:mCherry-NTR; LEN-P0.8:EGFP) hearts in Ctrl MO (B) and tnnt2a MO (C) injected groups at 24 hpt. (D) Quantification of the EGFP ratio of control and ablated hearts in Ctrl MO and tnnt2a MO injected groups at 24 hpt. N = 72, 85, 62, 59, respectively. Fisher?s Exact Test (2X3), ****, p < 0.0001. (E) Schematic timeline diagram of MTZ treatment and hemodynamic alteration. (F?H) Confocal stack projections of ablated Tg(vmhc:mCherry-NTR; LEN-P0.8:EGFP) hearts in Control (F), Tricaine (G), and BDM (H) treated groups at 24 hpt. (I) Quantification of the EGFP ratio of control and ablated hearts in control, Tricaine, and BDM treated groups at 24 hpt. N = 80, 145, 77, 236, 55, 137, respectively. Fisher?s Exact Test (2X3), **, p < 0.01, ****, p < 0.0001. (J) Quantification of the regeneration ratio of ablated hearts in control, Tricaine, and BDM treated groups at 96 hpt. N = 224 (160 strong + 64 weak EGFP signal), 107 (44 + 63), 50 (21 + 29), respectively. Chi-Square Test, *, p < 0.05, ***, p < 0.001, ****, p < 0.0001. Scale bars, 50 ?m. Dashed lines outline the hearts. dpf, days post fertilization; hpt, hours post treatment; MO, morpholino; MTZ, metronidazole.