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Figure 3 Transient analysis of enhancer activity for LEN motifs. (A) Schematic diagrams of full-length LEN, LEN-heart, LEN-heart with AP-1 mutation and LEN-fin elements. The original and mutated sequences of the predicted AP-1 binding site are also indicated. (B–E) Confocal stack projections of ablated hearts at 24 hpt from larvae injected with LEN (B), LEN-heart (C), LEN-heart AP-1 mut (D) and LEN-fin (E) constructs. (F) Quantification of the EGFP ratio of control and ablated hearts in LEN, LEN-heart, LEN-heart AP-1 mut and LEN-fin injected groups at 24 hpt. N = 28, 97, 30, 51, 30, 46, 48, 76, respectively. Chi-Square Test, ns, not significant, *, p < 0.05, ****, p < 0.0001. Scale bar, 50 μm. Dashed lines outline the hearts. hpt, hours post treatment.