Cone photoreceptors in cardiovascular disruption model systems. (A,C). Cryosections of doubly-transgenic (cdh5:gal4; UAS:nfsB-mCherry), DMSO-treated (DMSO Control; (A); Met-treated (Endothelial Cell-Depleted; (B); and Met-treated clutchmates (Met Control; (C) at 72 hpf, stained with zpr1, which labels red- and green-sensitive (LWS and RH2) double cones. Control retinas show numerous cones (A, arrow), while endothelial cell-depleted retinas display only a patch of cones in ventral retina (B). (D,E) Cryosections of normal clutchmates (D) and sih–/– embryos (E) at 72 hpf, stained with zpr1. Normal retinas show numerous zpr+ profiles while sih–/– retinas show a reduced number of cones. (F,G) Cryosections of normal clutchmates (F) and vlt–/– embryos (G) at 72 hpf, stained with zpr1, show similar patterns of staining. Scale bar (in B, applies to A–G) = 50 μm. (H,J) Assessment of distribution of zpr1+ cones, in retinas of endothelial cell-depleted vs. control embryos (H; p < 0.001, Fisher exact test; n = 7 for each condition), sih–/– vs. normal siblings (I; p < 0.001, Fisher exact test; n = 8 normal, 11 sih–/–), and vlt–/– vs. normal siblings (J; p = 1.0, Fisher exact test; n = 9 normal, 11 vlt–/–).
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