Retinal cell death in cardiovascular disruption model systems. (A,C) Cryosections of doubly-transgenic (cdh5:gal4; UAS:nfsB-mCherry), DMSO-treated (DMSO Control; (A); Met-treated (Endothelial Cell-Depleted; (B); and Met-treated clutchmates (Met Control; (C) at 72 hpf, stained with anti-activated caspase 3 (α-CC3). Control retinas show very few CC3+ profiles (arrow in A), while endothelial cell-depleted retinas display more widespread CC3+ stained-material (arrow in B), indicating cell death. (D,E) Cryosections of normal clutchmates (D) and sih–/– embryos (E), stained with α-CC3. Cell death appears not to be widespread in retinas of sih–/–. Scale bar (in A, applies to A–E) = 50 μm. (F,G) Quantification of CC3+ profiles in retinas of endothelial cell-depleted vs. control embryos (F; **p < 0.01, ANOVA with Tukey post hoc; n = 6 per condition) and sih–/– vs. WT embryos (G; p = 0.16, Student’s t-test; n = 6 per condition).
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