FIGURE 1

FIGURE 1

Ocular vasculature of cardiovascular disruption model systems. (A,D). Hyaloid and superficial vessels in zebrafish embryos doubly transgenic for cdh5:gal4 and UAS:nfsB-mCherry, treated with DMSO (controls; A,C), or metronidazole (Met; B,D), viewed at 48 h post-fertilization (hpf; A,B) and 72 hpf (C,D). The dorsal, nasal, and ventral radial vessels (drv, nrv, vrv, respectively) and hyaloid vasculature (hv) are visible in controls but not in Met-treated. (E,F) Hyaloid and superficial vessels of kdrl:eGFP transgenic zebrafish embryos, either normal (E) or sih–/– with no circulation and collapsed vessels (F). (G,H) Hyaloid and radial vessels of kdrl:eGFP transgenic zebrafish embryos, either normal (G) or vlt–/– with no erythrocytes but otherwise normal vasculature (H). Scale bar in A (applies to all) = 50 μm. 7-10 embryos were examined for each condition. All embryos were PTU-treated to prevent pigmentation from interfering with imaging.