IMAGE

FIGURE 4

ID

ZDB-IMAGE-210428-50

Genes

GFP
pappaa

Publication

Li et al., 2021 - Calcium State-Dependent Regulation of Epithelial Cell Quiescence by Stanniocalcin 1a

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Figures for Li et al., 2021

Image

Figure Caption

FIGURE 4

Papp-aa and Igfbp5a are indispensible for Stc1a action in NaR cells. (A) Embryos of the indicated genotypes were raised in E3 embryo medium until 5 dpf. The mRNA levels of papp-aa were measured and normalized. n = 15–17 larvae/group. (B)Tg(igfbp5a: GFP) embryos were injected with stc1a targeting gRNAs and Cas9 mRNA at the 1-cell stage. Embryos were raised in E3 embryo medium. The injected embryos were treated with BMS-754807 (BMS, 0.3 μM), ZnCl₂ (8 μM), or Bastimastat (200 μM) from 3 to 5 dpf. NaR cells were quantified and shown. n = 20–39 larvae/group. (C,D) Progeny of papp-aa^+/–; Tg (igfbp5a: GFP) intercrosses (C) or igfbp5a^{– /–}; Tg (igfbp5a: GFP) intercrosses (D) were injected with stc1a targeting gRNAs and Cas9 mRNA. The injected embryos were raised and NaR cells were quantified at 5 dpf. Each larva was genotyped afterward. n = 5–19 larvae/group (C) and n = 39–43 larvae/group (D). (E,F) Larvae of the indicated genotypes were raised in E3 embryo medium and NaR cells were quantified at 5 dpf. n = 9∼78 larvae/group (E) and n = 4∼34 larvae/group (F).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol