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Li et al., 2021 - Calcium State-Dependent Regulation of Epithelial Cell Quiescence by Stanniocalcin 1a

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FIGURE 1

The expression of stc1a, but not other stc genes, is regulated by Ca²⁺ levels. (A–D) Wild-type zebrafish embryos were raised in embryo media containing the indicated Ca²⁺ concentration until 3 days post fertilization (dpf). The mRNA expression levels of stc1a(A), stc1b(B), stc2a(C), and stc2b(D) were determined by qRT-PCR and normalized by β-actin mRNA levels. Data shown are from 3 independent experiments, each containing 10–15 embryos/group. In this and all subsequent figures, data shown are Mean ± SEM unless stated otherwise. Different letters indicate significant differences between groups by one-way ANOVA followed by Tukey’s multiple comparison test (P < 0.05) unless stated otherwise. (E–H) Zebrafish of the indicated genotypes were raised in E3 embryo medium until 5 dpf. The mRNA levels of stc1a(E), stc1b(F), stc2a(G), and stc2b(H) were measured, normalized, and shown. Data shown are from 3 independent experiments, each containing 10–15 larvae/group. ***P < 0.001 by unpaired two-tailed t-test.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol