IMAGE

Figure 4

ID

ZDB-IMAGE-210424-5

Antibodies

Ab3-map1lc3b

Source

Figures for Inoue et al., 2021

Image

Figure Caption

Figure 4

Immunoelectron microscopy of larsb-knockout larvae under Tg[fabp10:mcherry] background. (A–C) Immunoelectron microscopy of the liver, skeletal muscle, and spinal cord of larsb^+/+ larva. (D–I) Immunoelectron microscopy of the liver, skeletal muscle, and spinal cord of larsb^−/− larva. The bottom row shows higher magnification images (G–I). Large vacuoles in the livers of larsb^−/− larvae (asterisks) were composed of numerous irregular membranous structures, which showed immunoreactivity against both Lc3b (black arrows) and mCherry (black arrowheads) (D,G). Scale bar: 5.0 µm for (A,D); 1.0 µm for B and E; 500 nm for (C,F,H); 100 nm for (G,I). AP: autophagosome, N: nucleus, Larsb: leucyl-tRNA synthetase, Lc3b: microtubule-associated protein 1A/1B-light chain 3.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.