Fig. 5

Fig. 5

A Schematic of SuperTOP Flash (STF) reporter co-cultivation assay in AGS cells. AGS cells transfected with STF reporter were co-cultivated AGS cells transfected with combinations of with Wnt8a, Vangl2, Ror2 and ΔN-Vangl2 plasmid. B AGS cell STF reporter activation at each condition (i–viii). Scale bar = 10 µm. C Relative STF reporter activation in cells when co-cultured with control; Wnt8a; Vangl2; Wnt8a/Vangl2; Wnt8a/ΔN-Vangl2, Wnt8a/Ror2/Vangl2; Wnt8a/Ror2/ΔN-Vangl2 and Wnt8a/Vangl2/Ror2^3I. (n = 5, 10, 5, 5, 5, 5, 5, 5 repeats). D AGS cell STF reporter activation after IRSp53^4k addition: (i–iii); control; Wnt8a/Vangl2; Wnt8a/Vangl2/IRSp53^4k. Scale bar = 10 µm. E Relative STF reporter activation in cells when co-cultured with control; Wnt8a; Vangl2; Wnt8a/Vangl2; Wnt8a/Vangl2/IRSp53^4k. (n = 20 biological repeats across two independent experiments). C, E Data represented as box and whisker plots. Whiskers define the minimum and maximum values. Bounds of box indicate the 25th and 75th percentile, center line indicates the median, C calculated with exclusive median. C Student’s t-test and E One-way ANOVA test plus Tukey’s post-hoc test. Statistical significance: p ≤ 0.05. Source data are provided as a Source Data file.