IMAGE

Figure 1

ID

ZDB-IMAGE-210424-2

Antibodies

Ab1-lars

Publication

Inoue et al., 2021 - Leucyl-tRNA synthetase deficiency systemically induces excessive autophagy in zebrafish

All Figures

Figures for Inoue et al., 2021

Image

Figure Caption

Figure 1

Construction of larsb-knockout mutant zebrafish line. (A) Diagram showing the larsb genomic locus, CRISPR/Cas9 target site, and larsb-knockout (larsb^−/−) zebrafish mutant genotype. The sgRNA target sequence is displayed in green and the PAM region in red. In the genomic sequencing analysis chromatograms, the deletion region in the mutant larsb^−/− zebrafish is shown by the red box. (B) The Lars protein of larsb^−/− zebrafish had a missing editing domain. Western blot analysis of the Larsb protein expression in larsb^+/+ and larsb^−/− zebrafish. β-actin levels served as the loading control. Larsb: leucyl-tRNA synthetase b.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.