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Fig. 2

ID: ZDB-IMAGE-210419-6
Publication: Don et al., 2021 - In vivo Validation of Bimolecular Fluorescence Complementation (BiFC) to Investigate Aggregate Formation in Amyotrophic Lateral Sclerosis (ALS)
All Figures: Figures for Don et al., 2021

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Fig. 2

Optimized mVenus fragment (VN155-I152L) increases the signal-to-noise ratio of TDP-43 complementation. a Representative microscope images demonstrating the fluorescence reconstitution in muscle tissue at 24 hpf using VN155-TDP-43 versus VN155-I152L-TDP-43 constructs. Scale bars represent 20 μm. b Quantitative comparison of signal-to-noise ratio using standard VN155 versus VN155-I152L fragments. Data was obtained using an unbiased plate reader. Data pooled and averaged from three independent replicates. c, d The background fluorescence (noise) was significantly reduced with the I152L mutation (c), while the maximum fluorescence intensity did not change (d). Data in c, d was obtained using an unbiased plate reader. Data pooled from three independent replicates. *P < 0.05; ****P < 0.0001; ^nsP > 0.05

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