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Fig. 6 The upstream and downstream relationship among the adenosine monophosphate‐activated protein kinase (AMPK) pathway in the zebrafish cardiomyocytes. (a,b) Quantitative polymerase chain reaction and western blot analysis of AMPK pathway in the cardiomyocytes transfected with short hairpin AMPK (shAMPK). Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) was measured as the control. (c,d) Quantitative polymerase chain reaction and western blot analysis of the AMPK pathway in the cardiomyocytes transfected with short hairpin p53 (shp53). GAPDH was measured as the control. (e,f) Quantitative polymerase chain reaction and western blot analysis of the AMPK pathway in the cardiomyocytes transfected with short hairpin klf2a (shklf2a). GAPDH was measured as the control. Each experiment was repeated a minimum of three times. *Statistically significant difference (P < 0.05), **Highly significant difference (P < 0.01) in a two‐tailed Student’s t‐test. p‐AMPK, phospho‐adenosine monophosphate‐activated protein kinase; STX, streptozocin.