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Fig. 5 Adenosine monophosphate‐activated protein kinase (AMPK)–p53–Krüppel‐like factor 2a (klf2a) pathway involved in hyperglycemic zebrafish cardiomyocytes. (a) Real‐time polymerase chain reaction and western blot analysis of the expression of AMPK in hyperglycemic and control zebrafish cardiomyocytes, and control with A769662 intervention in vitro. (b) Real‐time polymerase chain reaction analysis of the expression of p53 and klf2a in hyperglycemic and control zebrafish cardiomyocytes, and control with A769662 intervention in vitro. (c) Real‐time polymerase chain reaction analysis of the expression of glucose transporters (Glut) in hyperglycemic and control zebrafish cardiomyocytes, and control with A769662 intervention in vitro. (d) Terminal deoxynucleotidyl‐transferase‐mediated dUTP nick‐end‐labeling (TUNEL) assay analysis of cell apoptosis in hyperglycemic and control zebrafish cardiomyocytes, and control with A769662 intervention cardiomyocytes cultured in vitro; scale bars, 100 μm. Each experiment was repeated a minimum of three times. *Statistically significant difference (P < 0.05), **Highly significant difference (P < 0.01) in a two‐tailed Student’s t‐test. p‐AMPK, phospho‐adenosine monophosphate‐activated protein kinase; STX, streptozocin.