ZFIN Image: Castro et al., 2021, Figure 4

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Figure Caption

Figure 4

Finfold growth and gene expression in leo^t1/lof^dt2 mutants and controls (Wt). (A) Fin phenotype comparisons in adults. (B,C) Finfold size comparison during development evaluated after and1 ISH (B), followed by measurements (C), revealing a longer finfold detectable since 48hpf in leo^t1/lof^dt2 mutants, in comparison with controls (n = 20, *p < 0.05). (D) Gene expression analyses by RT-qPCR at 86hpf, suggest lower expression levels for hoxd13a and bmp2b in leo^t1/lof^dt2 fins in comparison with Wt controls (***p < 0.001) detected by RT-qPCR (on the right) and ISH (on the left), which was accompanied by decreased expression of casp3, involved in apoptosis (**p < 0.01) and ccnb1, involved in proliferation (***p < 0.001). Statistical significance evaluated by unpaired t-test.

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