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Fig 7
The cell viability in cells treated with MG for 24 h in the presence and absence of CQ for 16 h was determined using SRB assay (a). Apoptotic cells were quantified using Annexin V-FITC/PI staining and FACS analysis after 40 μg/ml MG treatment for 48h in the presence and absence of CQ for 16 h (b). The blockage of autophagy flux was confirmed by detecting the accumulation of LC3-II using western blot (c). Data are presented as the mean±SD of three independent experiments in triplicate, ** p<0.01).
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