ZFIN Image: Huang et al., 2021, Fig 6

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Figure Caption

Fig 6 Methyl Gallate (MG) treatment causes changes in autophagy- and apoptosis-related proteins.

HepJ5 cells were treated with 40 μg/ml MG or vehicle for 48h. a. Levels of autophagy-related proteins (ATG5, ATG12, Beclin-1, and LC3) were checked by western blot. Amounts of ATG5, ATG5+12, and Beclin-1 were similar between MG-treated and vehicle-treated samples. The ratio of LC3-II/LC I was high in the MG-treated sample. b. Levels of cell apoptosis-related proteins (Bcl-2, Bax, Bad, c-caspase 3 and c-PARP) were checked. MG-treated cells showed decreased Bcl-2 (antiapoptotic), increased Bax and Bad proteins (proapoptotic), and increased cleavage of caspase 3 (c-caspase3) and PARP (c-PARP). All experiments were repeated at least three times independently (** p<0.01).

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