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Fig. 5 qRT-PCR bar graphs showing the log2 fold change in the gene expression in UVB-exposed and IOP-treated UVB exposed groups (5 dpf) compared to control (set at 0). Significant upregulation was observed in GADD45, RAD51, XRCC5, and XRCC6 in the IOP-treated UVB-exposed group (Kruskal-Wallis test, H = 32.31, p < 0.001) compared to only UVB-exposed group. UVB exposed group showed significant upregulation of p53 and BAX genes (p < 0.0001) that indicated enhanced apoptosis and cell death leading in zebrafish embryos.
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