Fig. 1

Fig. 1

Propofol treatment results in microphthalmia and defects in retinal lamination and function. a Compared with control embryos, propofol-treated embryos (5 μg/ml propofol) at 2, 3, and 6 days postfertilization (dpf) display a reduction in eye size. b Measurements of absolute eye size along the anteroposterior (A/P) and dorsoventral (D/V) axes at 2, 3, and 6 dpf. n = 12. Error bars indicate the standard error of the mean. c Transverse histological sections of retinas of control and propofol-treated embryos (5 μg/ml propofol) at 2, 3, and 6 dpf. Eyes of propofol-treated embryos (5 μg/ml propofol) are smaller than control eyes, and the ganglion cell layer, inner nuclear layer, and outer nuclear layer are not morphologically identifiable in the retinas of propofol-treated embryos at 3 dpf. The dorsal side is up in all images. n = 7. d, e In response to prey, the extent of eye rotation is linearly associated with the initial position of the eye in control larvae at 6 dpf; in propofol-treated larvae (5 μg/ml propofol), there is no convergent eye movement. n = 35. a Scale bar, 100 μm. c Scale bar, 35 μm