FIGURE 8

FIGURE 8

Analysis of Neurotransmitters level in epileptic zebrafish brains after pre-treatment with anti-HMGB1 mAb. Concentration of GABA (A) and Glutamate (B) and GABA to Glutamate ratio (C) in the corresponding experimental groups. Data are represented as mean ± SEM, n = 6, and statistically analyzed by one-way ANOVA followed by Sidak’s multiple comparison test. ^&&&p < 0.001 represents the significance level when second hit PTZ group is compared with VC group. ***p < 0.001 and *p < 0.05 represents the significance level when second hit PTZ group is compared with Pilo group. ^###p < 0.001 and ^#p < 0.05 represents the significance level when second hit PTZ group is compared with PTZ group. ^$$$p < 0.001, ^$$p < 0.01 and ^$p < 0.05 represent the significance level when DZP, mAb 1, mAb 2.5, and mAb 5 treated groups is compared with second hit PTZ group.