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Figure 2. The effect of rutin on hepatic steatosis in zebrafish larvae exposed to ethanol. (a) Representative images of Oil Red O staining. Zebrafish larvae (4 dpf) were incubated with 2% ethanol and/or 1 μM rutin for 24 h and hepatic fatty acid accumulation was measured by Oil Red O density. Marked areas indicate the livers of larvae. Quantitative analysis of relative intensities of Oil Red O staining of larval livers is presented. (b) Zebrafish total RNA was analyzed to determine c/ebpα and pparγ expression. Data are shown as means ± SEM of measurements obtained from each of the 20 larvae based on 3 independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001.