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Figure 2 T-Rex-inducible expression in Hep3B cells resulted in TAp73β overexpression, with no detectable expression of DNp73 protein. Hep3B cells with (Hep3BTAp73β DOX+) or without (Hep3BTAp73β DOX−) doxycycline treatment were compared to H1299 transiently transfected with TAp73α (H1299-TAp73α) or DNp73 (H1299-DNp73). Western blot analysis of cell lysates using IHC00197 antibody recognizing TAp73 isoforms (top panel) and western blot analysis of cell lysates using ΔNp73-1.1 antibody recognizing DNp73 isoforms [31,32] (bottom panel). β-actin antibody was using as a loading control.
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