ADSCs induce the expression of the NF-kappa B molecules in CC cells. (a–f) The photographs show the expression of transcription factors of the NF-Kappa B family including RelB (a), p52 (c) and p65 (e) obtained by immunofluorescence of HeLa cells cultured with serum-free DMEM medium or ADSC-CM (free of serum) for 24 h. The images were taken under a confocal microscope. The scale bar = 60 μm. The cell nuclei were contrasted with DAPI. Each experiment was repeated at least three times and to quantify the expression levels of Relb (b), p52 (d), and p65 (f), the intensity of fluorescence was quantified using ImageJ. Graph represents three biological replicates, error bars are s.d. and ****p < 0.0001 and **p < 0.01.
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