Figure 4

Figure 4

ADSC increases the CSC population, migration and invasion in an in vivo model. (a) Images show that the migration capacity of SiHa cells increases proportionally with respect to the amount of ADSCs inoculated in zebrafish embryos after 12 h. SiHa cells are shown in green due to they were transfected with a plasmid harboring a GFP gene. The images show a gradual increase in the migration and invasion of cancer cells from the yolk to the tail of embryos due to the presence of ADSCs. The white arrows show the migration areas in the embryo. (b) The table shows the number of cells inoculated in zebrafish embryos and the proportion of tumors formed in each condition. SiHa, SiHa + ADSC, or control cells: ADSC, HaCaT + ADSC, SiHa + HaCaT and SiHa + NIH3T3 were inoculated into zebrafish embryos, and the tumors were monitored every day for 5 days. (c) The graph depicts the frequency of CSC in each condition representing the number of cells injected with respect to the logarithmic fraction of animals without tumors. (d) The table shows the frequency of CSC calculated based on the ELDA software. (e) The images show tumors developed during 5 days in zebrafish embryos inoculated with ADSC, SiHa or SiHa + ADSC cells. Each experiment was repeated at least three times. (f) The table shows the number of cells inoculated in zebrafish embryos and the proportion of tumors formed in embryos injected with CaSki cells or CaSki + ADSCs cells. (g) The table shows the frequency of CSC calculated based on the ELDA software. (h) The graph depicts the frequency of CSC in each condition representing the number of Caski cells injected with respect to the logarithmic fraction of animals without tumors. (i) The images show tumors developed during 5 days in zebrafish embryos inoculated with CaSki or CaSki + ADSC cells.