Figure 2

Figure 2

Deregulated genes altered by the presence of ADSC exhibit clinical significance in cervical cancer patients. (a–f) Kaplan Meyer curves comparing the overall survival of 304 cervical squamous cell carcinomas with low versus high expression of altered genes (ITGA5, IL6, IL4R, FN1, PLAC8, TIMP1). Data were obtained from a public database: KM Plotter. The “p” values are shown in each of the graphs. ADSCs do not alter the proliferative capacity of HeLa cells. (g) Cell cycle analysis of HeLa cells cocultured in the presence of ADSCs compared to HeLa control cells. (n = 3, the error bars are s.e.m) The graph shows no significant changes in any of the phases of the cell cycle. (h) Proliferation assay of HeLa control vs HeLa cells cocultured at different times. (i) The picture shows the method to obtain the conditioned medium of ADSCs (ADSC-CM) or the conditioned medium of HeLa cells (HeLa CM). ADSCs or HeLa cells were cultured in serum-free DMEM for 24 h and then the conditioned medium was employed to culture CC cells. (j) Proliferation assay of HeLa cells cultured with different conditioned media (CM) at different times. (n = 3, the error bars are S.D., but no significant changes were shown).