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Fig 3

ID: ZDB-IMAGE-210307-41
Publication: Belmonte et al., 2021 - son is necessary for proper vertebrate blood development
All Figures: Figures for Belmonte et al., 2021

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Figure Caption

Fig 3 <italic>son</italic> knockdown causes a reduction in neutrophils.

(A) Representative images of mpx:GFP embryos at 48hpf that were uninjected (left) or MO-injected (son MO, right). Images are taken at 40x, and every green dot is a labelled neutrophil; these cells are in tissues and not circulating. (B) Each point represents the number of GFP+ myeloid cells present in an individual uninjected, MO-injected (son MO), or MO-injected with son RNA (rescue) embryo. Images were taken and GFP⁺ cells were manually enumerated. Middle lines represent mean and error bars represent SD. (C) qRT-PCR for mpx (left) and csf3r (right) was performed. Each point represents ten embryos randomly selected from uninjected, MO-injected (son MO), or MO-injected with son RNA (rescue) conditions that were analyzed by qRT-PCR. Middle lines represent mean and error bars represent SD. * represents p = 0.02, ** represents p = 0.01, *** represents p < 0.001, N.S. represents no significance.

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