Figure 4

Figure 4

In vivo uncaging of pcHoechst with optimized light intensity at single‐cell resolution. H2B‐mRFP (red) injected zebrafish embryos were incubated with 100 μM pcHoechst in the dark overnight and activated by using a 405 nm UV laser at 0.07 mW and at 74 hpf. The arrow indicates a nucleus targeted with two bleach points. A pre‐bleach image was recorded prior to illumination, post bleach images 7.5 min and 1.5 h after bleaching. The white rectangles depict magnified areas. Uncaged pcHoechst (blue) can be observed 7.5 min and 1.5 h after UV illumination and colocalizes with H2B‐mRFP (red) in a single nucleus. Representative images of two experiments with the same intensity, and ten experiments in total.