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Fig 4 (A and B) EPC cells were seeded into 24-well plates overnight and co-transfected with MAVS-, MITA-, or TBK1-expressing plasmid and empty vector or TMEM33-Myc (250 ng or 250/500 ng), plus IFN?1pro-Luc (A and C) or ISRE-Luc (B and D) at the ratio of 1:1:1. pRL-TK was used as a control. At 24 h post-transfection, cells were lysed for luciferase activity detection. Data were expressed as mean ± SEM, n = 3. Asterisks indicate significant differences from control (*, p < 0.05).
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