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Figure 5 5-Bromoprotocatechualdehyde (BPCA) isolated from Polysiphonia japonica protects against PA-induced β-cell dysfunction in zebrafish. (A) Zebrafish were incubated with 50 µM BPCA and 0.2 mM PA from 3 to 4 days post-fertilization (dpf). BPCA was added 1 h prior to PA treatment. Thereafter, the zebrafish were incubated with 3 or 20 mM glucose for 3.5 h. (B) Confocal microscopy images of the pancreata of the zebrafish. Scale bar: 100 µm. (C). Relative EGFP fluorescence levels from (B). (D) Numbers of β-cells per embryo from (B). n = 4~6. * p < 0.05, ** p < 0.005, *** p < 0.001, n.s. = no significance.