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Figure 2 5-Bromoprotocatechualdehyde (BPCA) isolated from Polysiphonia japonica protects PA-induced cell death in Ins-1 cells. Ins-1 cells were incubated with 50 µM BPCA for 1 h and then further incubated with/without 0.2 mM PA for 24 h. (A) Further incubation with Hoechst 33342 (HO342, 10 µg/mL) subsequently performed in the images. (B) Further incubation with propidium iodide (PI, 50 µg/mL) subsequently performed in the images. Scale bar: 10 µm. (C). Western blotting was subsequently performed as described in “Materials and Methods”. * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. = no significance.
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Full text @ Antioxidants (Basel)