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Fig. 4 Fig. 4. Intestinal β-catenin is increased with rhRSPO1 at 14 days. Immunofluorescent staining of proximal intestinal segments for bromodeoxyuridine (BrdU) for Sham + PBS(A), SBS + PBS(B), Sham + RSPO1(C), and SBS + RSPO1(D) fish at POD14. Quantification of BrdU + cells/villus fold(E). Immunofluorescent staining of proximal intestinal segments for β-catenin for Sham + PBS(F), SBS + PBS(G), Sham + RSPO1(H), and SBS + RSPO1 fish(I) at POD14. Quantification of β-catenin + cells/villus fold(J). Scale bars 100 μm *p < 0.05, **p < 0.01.
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