Fig. 2

Fig. 2 Fig. 2. A small but significant portion of Zcdx1b−/− mutant embryos exhibited absent pitx2c expression in the left dorsal diencephalon. A The nucleotide sequences and protein domains of wild-type and Zcdx1b−/− mutants are shown. Deleted nucleotides are indicated by a dashed line, while inserted nucleotides are shown in red lettering. The sgRNA target site in the activation domain is highlighted in yellow and the protospacer adjacent motif (PAM) sequence is shown in blue. B Nucleotide sequences of wild-type (WT) and Zcdx1b−/− mutants (MT) within the sgRNA target region are shown. C Percentages of embryos with left, right, bilateral or absent expression patterns of pitx2c in the dorsal diencephalon from intercross of Zcdx1b+/- F2 mutants are shown. Data were combined from two different intercrossing experiments. Chi-squared test of independence was conducted to compare portions of embryos with absent pitx2c expression in the dorsal diencephalon between sibling wild-type and Zcdx1b+/- or Zcdx1b−/− mutant embryos. ∗, p ​< ​0.05.